Liquid chromatography dual zone packing materials
US4778600A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 17, 1987 |
| Grant date | Oct 18, 1988 |
| Priority date | — |
| Expiry date | Jun 17, 2007 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J20/3263
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Dual zone reverse phase packing materials for use in liquid chromatographic blood serum analysis are prepared by contacting a porous support, such as porous silica, with a fluorocarbon silane, such as a perfluorobutylsilyl compound, to form a lipophobic phase in the external zone of the porous support, and, then contacting the porous support with a lipophilic silane, such as an octadecylsilyl compound, to form a lipophilic partitioning phase in the internal zone of the porous support. Ketal blocked diol groups, which later may be hydrolyzed to diol groups, are also attached to both zones of the packing materials. The dual zone reverse phase packing materials display a reduced degree of serum protein adsorption in the external zone while drug substances are retained and separated by the lipophilic partitioning phase in the internal zone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.