Carbonic anhydrase inhibitor-tagged nucleic acid probes
US4780405A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 9, 1985 |
| Grant date | Oct 25, 1988 |
| Priority date | — |
| Expiry date | Jul 9, 2005 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/532
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Nucleic acid hybridization probes are provided which comprise nucleoside bases or terminal nucleotide phosphates chemically linked to aromatic sulfonamide inhibitors of carbonic anhydrase. Methods of preparing probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. A probe of the invention is detected by binding to it a reporter group, such as a homopolymer or heteropolymer of enzymes, which includes a carbonic anhydrase which binds to the inhibitor linked to the probe, and then detecting the bound reporter group, as by production of a fluorescent or colored product in a reaction catalyzed by an enzyme component of the reporter group. Also provided are enzyme immunoassays wherein detection of antibody is by a process which comprises a chemical reaction catalyzed by a carbonic anhydrase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.