Recombinant DNA expression vectors useful in bacillus and other host cells
US4783405A · kind A · utility
10Cited by
1References
87Claims
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Key dates
| Filing date | Sep 26, 1984 |
| Grant date | Nov 8, 1988 |
| Priority date | — |
| Expiry date | Sep 26, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/76
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant DNA expression vectors for use in Bacillus and other host cells are disclosed. The vectors comprise the veg promoter sequence of Bacillus subtilis, a novel ribosome binding site-containing sequence and a sequence that codes for a functional polypeptide. The ribosome binding site-containing sequence is synthesized in accordance with conventional procedures while the veg promoter sequence can be obtained from E. coli K12 JA221/pMS480 (NRRL B-15258). Various sequences that codes for functional polypeptides and method for their expression in Bacillus are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.