Gene coding for signal peptides and utilization thereof
US4783415A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 27, 1985 |
| Grant date | Nov 8, 1988 |
| Priority date | — |
| Expiry date | Mar 27, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A DNA segment of a gene coding for a signal peptide is disclosed the segment is to be used in a host-vector system employing a bacterium belonging to the genus Streptomyces as a host by incorporation in the vector DNA. The segment comprises a DNA having a base sequence coding for the peptide having the amino acid sequence from X to Y in the annexed FIG. 1. A vector plasmid to be used for transformation of bacteria belonging to the genus Streptomyces is also disclosed. The plasmid comprises a DNA segment with a base sequence coding for an extra-cellular .alpha.-amylase derived from Streptomyces hygroscopicus SF-1084 strain or a mutant thereof the DNA segment incorporated into a vector plasmid is to be used in a host-vector system employing a bacterium belonging to the genus Streptomyces as a host and also having a DNA segment necessary for expression of the gene information of the DNA segment. The present invention may be applied to the production of a desired protein which will be secreted outside the host microorganism. .
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.