Expression of factor VII activity in mammalian cells

US4784950A · kind A · utility

Assignee

• ZYMOGENETICS INC. · US

Inventors

• Frederick S. Hagen · Seattle, US
• Mark J. Murray · Sugar Land, US
• Sharon J. Busby · Des Moines, US
• Kathleen L. Berkner · Cleveland, US
• Margaret Y. Insley · Des Moines, US
• Richard Woodbury · Dover, US
• Charles L. Gray, Jr. · Pinckney, US

Key dates

• Filing date: Dec 16, 1986
• Grant date: Nov 15, 1988
• Expiry date: Dec 16, 2006

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/20
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. In particular, the first nucleotide sequence may be derived from a genomic clone or cDNA clone of Factor VII. The second sequence encodes a catalytic domain for the serine protease activity of Factor VIIA. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as Factor VIIa.