Isolation of fibroblast growth factor

US4785079A · kind A · utility

Assignee

• Salk Institute for Biological Studies · US

Inventors

• Denis Gospodarowicz · San Francisco, US
• Andrew Baird · San Diego, US
• Peter Bohlen · Uster, CH

Key dates

• Filing date: Dec 10, 1986
• Grant date: Nov 15, 1988
• Expiry date: Dec 10, 2006

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/503
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.