Factor IX therapeutic blood product, means and methods of preparing same
US4786726A · kind A · utility
28Cited by
7References
11Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 22, 1987 |
| Grant date | Nov 22, 1988 |
| Priority date | — |
| Expiry date | May 22, 2007 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of producing highly purified factor IX from crude factor IX comprising binding an A-7 monoclonal antibody to an Affigel-10 column; partially purifying the crude factor IX to produce a partially purified factor IX; applying that factor IX to the A-7-Affigel-10 column with an application buffer; washing the column with 1-3M NaCl in 0.05M Tris-HC1, 20 mM MgCl.sub.2 and 1 mM Benzamidine HCl; eluting purified factor IX from the washed column with 20 mM EDTA; and separating purified factor IX from the EDTA. An improved factor IX product produced thereby.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.