Patent · US Expired

System for efficient isolation of genes using probes

US4788135A · kind A · utility

40Cited by

1References

2Claims

0Family size

Assignee

The Board of Trustees of the Leland Stanford Jr. University · US

Inventors

Ronald W. Davis · Menlo Park, US
Richard A. Young · Weston, US

Key dates

Filing date	Oct 10, 1985
Grant date	Nov 29, 1988
Priority date	—
Expiry date	Oct 10, 2005

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/6854
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Systems are provided for the dual purpose of cloning genes using antibodies as probes and isolating unknown proteins encoded by cloned DNA. The method employs a vector derived from phage which is used in combination with a high frequency lysogenic host. The vector is further characterized by having an inducible promoter regulating expression of a gene into which the foreign DNA may be introduced to produce a fused protein, and controlled induction of the prophage with rapid increase in copy number and high level transcription of foreign DNA. The technique is exemplified with a specific lambda phage construct in conjunction with the .beta.-galactosidase structural gene lacZ.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.