Platelet aggregation reagent, reagent container and method of determining platelet aggregation in EDTA-anticoagulated blood
US4788139A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 6, 1987 |
| Grant date | Nov 29, 1988 |
| Priority date | — |
| Expiry date | Aug 6, 2007 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/86
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A platelet aggregation reagent for EDTA-anticoagulated blood comprises calcium chloride, sodium citrate and a platelet-aggregating agent; the calcium chloride and the sodium citrate being present in a proportion of about 1.5 micromoles: 5 micromoles to about 7.5 micromoles: 2.5 micromoles per at least about a platelet aggregating amount of the agent. A platelet aggregation reagent container for EDTA-anticoagulated blood comprises an elongated container suitable for use in a platelet counter containing a reagent comprising about 1.5 micromoles to 7.5 micromoles of calcium chloride, about 2.5 micromoles to 5.0 micromoles of sodium citrate, and at least about a platelet aggregating amount of a platelet aggregating agent. A method of determining platelet aggregation in EDTA-anticoagulated blood, comprises counting the number of platelets contained in an EDTA-anticoagulated blood sample to obtain an initial platelet count (IC), contacting the sample with calcium chloride, sodium citrate and a platelet-aggregating agent in proportions of at least about a platelet aggregating amount of the agent per about 450 microliters of the samples and about 1.2 micromoles to 7.5 micromoles of the cal…
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