Homogenous specific binding assay reagent system and labeled conjugates
US4791055A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 1986 |
| Grant date | Dec 13, 1988 |
| Priority date | — |
| Expiry date | Jan 9, 2006 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/581
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.