Non-enzymatic immunohistochemical staining system and reagents
US4792521A · kind A · utility
31Cited by
4References
25Claims
0Family size
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Inventor
Key dates
| Filing date | Aug 15, 1985 |
| Grant date | Dec 20, 1988 |
| Priority date | — |
| Expiry date | Aug 15, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A non-enzymatic method of immunohistochemical staining uses a bound electron transfer agent to achieve amplification of antibody binding to specific antigens of interest in a histology or cytology specimen. A preferred embodiment uses a bound phenazine, which is reduced with a soluble reducing agent, and which in turn reduces a tetrazolium salt, to precipitate a formazan dye at the site of primary antibody binding to the antigen of interest. Reagents and kits for use in the method are also provided.
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