Determination of hydrogen peroxide using fluorogenic dihydroquinolone and dihydrocoumarin indicators
US4794079A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 30, 1985 |
| Grant date | Dec 27, 1988 |
| Priority date | — |
| Expiry date | Sep 30, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2326/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Fluorogenic indicator compounds responsive to the presence of hydrogen peroxide and methods and test compositions for determining hydrogen peroxide in a test sample. Preferred indicator compounds are 3,4-dihydrocoumarin derivatives and 3,4-dihydro-2-quinolone derivatives, especially those having a dialkylamino group at the 7-position. Such particularly preferred compounds are of the formula: ##STR1## wherein R.sup.1 is hydrogen, cyano, or --COOR.sup.6, --CONHR.sup.6 or --CON(R.sup.6).sub.2 where R.sup.6 is hydrogen, alkyl, alkenyl, or aryl; R.sup.3 and R.sup.4 are lower alkyl; and Y is .dbd.0 or .dbd.N--R.sup.5 where R.sup.5 is hydrogen or lower alkyl. The indicator compounds yield highly fluorescent products upon oxidation by hydrogen peroxide in the presence of a peroxidatively active substance and are useful in analytical systems which generate hydrogen peroxide in response to an analyte under determination in a test sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.