Method for sequential centrifugal stratification chromatography for separating components from mixtures
US4797215A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 4, 1986 |
| Grant date | Jan 10, 1989 |
| Priority date | — |
| Expiry date | Feb 4, 2006 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/381
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In the new on-line preparative method, and in the inventive apparatus, the addition of flow medium during chromatography can be varied in parallel, locally and temporally. It is thus possible to improve sections of a total chromatogram in their separation by choosing for such partial problems the optimum compositions of the flow medium. The separations are basically obtained in two different ways: (1) Circular separations are carried out with the help of centrifugal force using a sequential technical flow medium supply, wherein the separation occurs from the inside to the outside. The separated substances can be returned through recycling to the inside of the layer. (2) Anticircular separations are carried out with the help of capillary force using a circular solvent supply, wherein the substance zones are concentrated and/or further developed from the outside to the inside. The circular and anticircular possibilities of use can be combined as desired, so that the separation path can be extended practically without limit and thus the capacity and separation performance can be significantly increased.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.