Methods for attaching polynucleotides to supports
US4797355A · kind A · utility
67Cited by
2References
3Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 13, 1985 |
| Grant date | Jan 10, 1989 |
| Priority date | — |
| Expiry date | Jun 13, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6813
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A probe is attached to a stock support to which is bound a single-stranded polynucleotide by hybridizing both the probe and the bound polynucleotide to a connecting strand with which they are both complementary and then ligating an end of the bound polynucleotide and the probe. After denaturation, the probe remains attached to the support by way of the bound polynucleotide. A target polynucleotide is then hybridized with the support-bound probe and with a labelled probe to provide a detectable hybridization complex.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.