Compound 1-(3-(2-pyridyldithio)propionamido)-12-(5-hydrazidoglutaramido)-4,9-diox adodecane

US4797491A · kind A · utility

Assignee

• Cetus Corporation · US

Inventors

• Danute E. Nitecki · Berkeley, US
• Margaret Moreland · Berkeley, US

Key dates

• Filing date: Mar 17, 1986
• Grant date: Jan 10, 1989
• Expiry date: Mar 17, 2006

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54353
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Bifunctional chemicals useful for linking aldehyde containing moieties to moieties containing functionalities which are or can be reactive with sulfhydryl are claimed and have the formula: EQU L--S--(CH.sub.2).sub.n1 --CONH--SPACER--NH--X wherein L is a leaving group selected from --H or --S--Ar, wherein Ar represents optionally substituted phenyl or pyridyl; PA0 n.sub.1 =2-4; PA0 X is selected from: PA0 --CO--Y--CONHNH.sub.2, a hydrazide; ##STR1## a hydrazine; ##STR2## a hydrazine; --CO--Y--NH--CONHNH.sub.2, a semicarbazide; and PA0 --CO--Z--NH--CSNHNH.sub.2, a thiosemicarbazide; PA0 wherein Y is alkylene or oxaalkylene and Z is alkylene or a polypeptide residue bridging the N-terminal amino group and C-terminal carboxy group thereof; PA0 the SPACER is oxaalkylene or oxaalkylene substituted with hydroxyl and specifically includes residues having formulas selected from EQU --(CH.sub.2).sub.n2 --O--(CH.sub.2).sub.n2 --O--(CH.sub.2).sub.n2 --(a) PA0 wherein each n2 is independently 2-4 and ##STR3## wherein n3 is 2-6. Also claimed are hydrazides, semicarbazides, and thiosemicarbazides of .omega.-mercaptocarboxylic acids which lack SPACER diamine.