Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US4801529A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 18, 1985 |
| Grant date | Jan 31, 1989 |
| Priority date | — |
| Expiry date | Jun 18, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed for isolating a mutant microorganism. The method includes the following steps: PA0 (1) separately microencapsulating individual or small numbers of a microorganism population containing the mutant to obtain a first microdroplet; PA0 (2) thereafter surrounding the first microdroplet, with or without an outer semi-permeable membrane, with an outer gel-coating to obtain a gel-coated microdroplet, wherein the gel layer contains an indicator material which reacts to the presence of the mutant microorganism; PA0 (3) culturing the resultant microcapsules so that the microdroplets containing mutant microorganism are able to induce a detectable difference in the indicator material from microdroplets, containing non-mutant microorganisms; and PA0 (4) separating the microdroplets containing mutant microorganisms from those containing non-mutant microorganisms based upon the differences in the indicator material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.