Repetitive hit-and-run immunoassay and stable support-analyte conjugates; applied to T-2 toxin
US4801726A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 1986 |
| Grant date | Jan 31, 1989 |
| Priority date | — |
| Expiry date | Apr 15, 2006 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56911
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A repetitive immunoassay analytical method for determination of a free analyte is carried out by loading an affinity column of covalently bound analyte with tagged antibody, passing a continuous aqueous stream of carrier liquid over the column, introducing an aliquot of a sample to be analyzed for free analyte into the carrier stream upstream of the column, and monitoring the eluting carrier stream for a signal spike resulting from the presence of tagged antibody material released from the column by the application of free analyte in the analytical sample. Many samples may be analyzed by this method before the antibody-loaded affinity column needs to be regenerated. It is also disclosed that substrate-analyte conjugates of superior stability are produced by linking a substrate to a hydroxyalkyl analyte via an amino, hydrazide, or sulfide linking group replacing a hydroxy group. Such stable substrate-analyte conjugates are useful in the production and purification of antibodies, as well as in the repetitive immunoassay of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.