Peptide-beta-lactamase conjugates for enzyme-linked immunoassays
US4803156A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Sep 11, 1985 |
| Grant date | Feb 7, 1989 |
| Priority date | — |
| Expiry date | Sep 11, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/31
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A reagent for an ELISA determination of an antibody, the reagent comprising a peptide covalently linked to beta-lactamase. The reagent can be used in the following method to detect antibodies in a sample which involves PA1 a. contacting the sample with protein A linked to a solid support, PA1 b. incubating the sample-protein A linked to the solid support, PA1 c. washing the incubated sample-protein A linked to the solid support, PA1 d. contacting the washed sample-protein A with the reagent, PA1 e. incubating the sample-protein A and reagent, PA1 f. washing the incubated sample-protein A-reagent, and PA1 g. determining the enzymatic activity of the resultant mass.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.