Hydrolyzable fluorescent substrates for phosphatases and analytical use thereof
US4803157A · kind A · utility
2Cited by
3References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 31, 1986 |
| Grant date | Feb 7, 1989 |
| Priority date | — |
| Expiry date | Jan 31, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Hydrolyzable substrates for acid and alkaline phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above about 530 nm and maximum fluorescent emissions at least about 580 nm. The dyes are blocked prior to hydrolysis with a phosphono or thioxophosphono group or a salt thereof. These substrates can be used in analytical determinations of acid or alkaline phosphatase, or in competitive binding reactions to determine immunologically reactive substances.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.