Nif promoter of fast-growing rhizobium japonicum
US4803165A · kind A · utility
6Cited by
1References
21Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Aug 7, 1985 |
| Grant date | Feb 7, 1989 |
| Priority date | — |
| Expiry date | Aug 7, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/743
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The promoter of the nifH gene of the fast-growing Rhizobium japonicum strain USDA 191, has been cloned. Over 4.2 kilobase pairs (kbp) of DNA were sequences (FIG. 1). Sequences encoding nifH and the 5'-end of nifD were identified, as were sequences involved in promoting operon transcription and a nifH ribosome binding site. Use of the nifH promoter to drive transcription in Rhizobium of heterologous structural genes is taught. Useful sequences and plasmids are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.