Hollow fiber cell culture device and method of operation
US4804628A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 19, 1987 |
| Grant date | Feb 14, 1989 |
| Priority date | — |
| Expiry date | Aug 19, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M41/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved cell culturing device includes a hollow fiber cartridge having a shell and a plurality of capillaries extending through the shell with at least some of the capillaries having selectively permeable walls. A cell culturing space is located between the shell and capillaries. The improvement includes a chamber containing a second medium supply fluidly connected to the cell culturing space. A pressurizing system pressurizes the medium within the chamber to a level higher than the level of medium flowing through the lumen of the capillaries. A valving mechanism alternatively and selectively restricts flow of medium between the chamber and the cell culturing space through first and second conduits such that circulation is effected in the cell culturing space.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.