Immobilization of nucleic acids on solvolyzed nylon supports
US4806631A · kind A · utility
15Cited by
6References
7Claims
0Family size
Assignee
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Key dates
| Filing date | Sep 30, 1985 |
| Grant date | Feb 21, 1989 |
| Priority date | — |
| Expiry date | Sep 30, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.