Double-labeled enzymeimmunoassay methods
US4808521A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 22, 1986 |
| Grant date | Feb 28, 1989 |
| Priority date | — |
| Expiry date | Jan 22, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method of performing an immunoassay of a ligand in a liquid sample wherein two independently measureable enzyme labels are separately conjugated to two or more components or populations of components of the assay system and, after completion of the complexing reaction, substantially all of the first enzyme label and a proportion of the second enzyme label are removed from the assay mixture, the proportion of the second label removed being related to the amount of the said ligand and the assay determined from a measurement of said proportion of the second label being normalised by comparison with a measurement of the total first label removed. In this way, manual pipetting errors and other assay procedural errors can be corrected for.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.