Analytical element and method for the determination of creatinine or creatine
US4812399A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 1986 |
| Grant date | Mar 14, 1989 |
| Priority date | — |
| Expiry date | Apr 21, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An analytical element can be used for the determination of either creatinine or creatine or both. The element contains creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, and a leuco dye which is capable of providing a detectable dye in the presence of hydrogen peroxide and a peroxidative substance. The creatine amidinohydrolase is present in a manner such that it is substantially inert to the leuco dye. The creatinine amidohydrolase is present in a rate limiting amount. By measuring the amount of dye formation at particular times during the assay, either or both of the analytes can be determined with the same element.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.