Multichain polypeptides or proteins and processes for their production
US4816397A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 14, 1984 |
| Grant date | Mar 28, 1989 |
| Priority date | — |
| Expiry date | Nov 14, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/24
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Multichain polypeptides or proteins and processes for their production in cells of host organisms which have been transformed by recombinant DNA techniques. According to a first aspect of the present invention, there is provided a process for producing a heterologous multichain polypeptide or protein in a single host cell, which comprises transforming the host cell with DNA sequences coding for each of the polypeptide chains and expressing said polypeptide chains in said transformed host cell. According to another aspect of the present invention there is provided as a product of recombinant DNA technology an Ig heavy or light chain or fragment thereof having an intact variable domain. The invention also provides a process for increasing the level of protein expression in a transformed host cell and vectors and transformed host cells for use in the processes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.