Patent · US Expired

Blood partitioning method

US4818418A · kind A · utility

53Cited by
19References
14Claims
0Family size

Assignee

Inventor

Key dates

Filing dateFeb 3, 1988
Grant dateApr 4, 1989
Priority date
Expiry dateFeb 3, 2008

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25375
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

The present invention is directed to a method for separating mononuclear cells from other cells in a blood sample which is more rapid than the currently used density gradient material and which provides a better separation than methods utilizing thixotropic, gel-like material. In the method, a water soluble density gradient material is placed in a centrifuged tube. A water insoluble, thixotropic, gel-like substance is also placed in the container. A blood sample is placed in the container and the container is centrifuged for a time during which various components interchange positions and the gel-like substance forms a barrier between the mononuclear cells and the other cells of the blood sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.