Amplified expression vector

US4820642A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jeffrey C. Edman · Davis, US
• William J. Rutter · San Francisco, US
• Robert A. Hallewell · San Francisco, US
• Pablo D. T. Valenzuela · San Francisco, US
• Howard Goodman · Newtown, US

Key dates

• Filing date: Apr 4, 1983
• Grant date: Apr 11, 1989
• Expiry date: Apr 4, 2003

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2730/10122
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A DNA expression vector which contains the trp promotor is described. The expression vector provides for the overproduction of .beta.-lactamase. Insertion of a gene or cDNA into the .beta.-lactamase gene of the expression vector results in the over-production of a fusion protein comprising a part of the .beta.-lactamase as the N-terminal end and the protein coded for by the inserted DNA as the C-terminal end. Using the expression vector described herein, it is possible to obtain large amounts of the fusion protein. A fusion protein containing the surface antigen of Hepatitis B virus and a vaccine containing this fusion protein are also described.