Method for increasing the sensitivity of nucleic acid hybridization assays
US4824776A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 25, 1985 |
| Grant date | Apr 25, 1989 |
| Priority date | — |
| Expiry date | Jul 25, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improvement in the sensitivity of hybridization assays for detecting polynucleotide target sequences is provided by selective dehybridization of the bound portion of the probe, separation of the dehybridized portion from the support and the residual sample, and concentrating the separated probe before detecting its presence by means of its reporter group. The concentration step can be carried out by adsorption of the dehybridized probe onto a basic ion exchange material. A displacer sequence having greater binding affinity for the target polynucleotide sequence may be used to dehybridize the bound probe portion for subsequent concentration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.