DNA base sequence, method for preparing a recombinant plasmid including the DNA base sequence, and breeding method for enhancing the protein-secreting ability of a microorganism by introducing thereinto the recombinant plasmid
US4824782A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Apr 8, 1988 |
| Grant date | Apr 25, 1989 |
| Priority date | — |
| Expiry date | Apr 8, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.