Avian retrovirus-bovine growth hormone DNA
US4828987A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 14, 1984 |
| Grant date | May 9, 1989 |
| Priority date | — |
| Expiry date | May 14, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/11043
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant DNA molecules in which an avian retroviral long terminal repeat (LTR) has been ligated to the bovine growth hormone gene are described herein. The retroviral LTR was derived from a plasmid clone of a Schmidt Rupin strain of Rous Sarcoma Virus while the bovine growth hormone (BGH) gene was derived from a lambda-bacteriophage genomic library. Using a transient eucaryotic expression assay system, the plasmid constructs were screened for their ability to direct expression and secretion of bovine growth hormone. These constructs were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.