Method for purifying an interferon
US4828990A · kind A · utility
Inventors
Key dates
| Filing date | Oct 10, 1986 |
| Grant date | May 9, 1989 |
| Priority date | — |
| Expiry date | Oct 10, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/142
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of purifying a polypeptide having a physiological activity such as one having interferon activities from a culture mixture of a microorganism obtained by a recombinant DNA technique and capable of producing the polypeptide is disclosed. The method comprises subjecting the cultured cells to extraction and purification in the presence of a salt of zinc or copper and polyethyleneimine thereby inhibiting decomposition and denaturation of the polypeptide. The extracted polypeptide can be further purified by column chromatographies using a column containing an anion exchange resin, column containing a cation exchange resin and column containing a gel filtration resin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.