Roller bottle system
US4829004A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 24, 1987 |
| Grant date | May 9, 1989 |
| Priority date | — |
| Expiry date | Nov 24, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M27/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An insert for a roller bottle of the type used in cell culture for vaccine manufacture is formed of a polymeric resilient material whereby it can be bent or rolled so that it can pass through the opening of the roller bottle. Once the insert is inside the bottle, it is released so that its resilient characteristic causes it to unbend or unroll. The insert is wider than the diameter of the roller bottle, and therefore it is urged against the interior wall of the roller bottle. Apertures and/or notches are provided in the insert to increase the turbulence of a fluid and to decrease the fluidic shear forces in the roller bottle as it is rotated in conventional operation. A significant increase in productivity is achieved because particles, which may be microcarrier beads, are maintained in suspension, and prevented from sinking to the bottom of the fluid medium where cells cannot grow. The roller bottle system is thereby transformed into a microcarrier suspension culture system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.