Process for the rapid and simple isolation of nucleic acids
US4830969A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 31, 1981 |
| Grant date | May 16, 1989 |
| Priority date | — |
| Expiry date | Aug 31, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/82
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the separation from other cellular materials of heat agglomeration resistant water soluble nitrogen containing organic compounds such as plasmids, RNA's, mitochondrial DNA's, viral DNA's, chloroplast DNA's, other episomal DNA's and certain proteins. The process comprises heating cellular materials in a solution of lysing agent to lyse the desired cells and to agglomerate water soluble nitrogen containing compounds such as certain chromosomal DNA's which are not resistant to agglomeration; centrifuging the resulting product to remove water soluble agglomerated materials; separating the supernatant liquid and precipitating the water soluble agglomeration resistant organic compounds with a water soluble precipitant. The process also includes separating the agglomeration resistant water soluble nitrogen containing compounds from each other by means of exclusion chromotography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.