Human serum albumin crystals and method of preparation
US4833233A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 20, 1987 |
| Grant date | May 23, 1989 |
| Priority date | — |
| Expiry date | Aug 20, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/31768
- WIPO fieldSurface technology, coating
- WIPO sectorChemistry
Abstract
HSA crystals are provided in the form of tetragonal plates having the space groups P42.sub.1 2, the crystals being grown to sizes in excess of 0.5 mm in two dimensions and a thickness of 0.1 mm. Growth of the crystals is carried out by a hanging drop method wherein a precipitant solution containing PEG and a phosphate buffer is mixed with an HSA solution, and a droplet of mixed solution is suspended over a well of precipitant solution. Crystals grow to the desired size in 3 to 7 days. Concentration of reagents, pH and other parameters are controlled within prescribed limits. The resulting crystals exhibit a size and quality such as to allow performance of x-ray diffraction studies and enable the conduct of drug binding studies as well as genetic engineering studies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.