Process and reagent for increasing the quantum yield in chemiluminescent reactions

US4834918A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Karl Wulff · Weilheim, DE
• Marin Berger · Weilheim in Oberbayern, DE

Key dates

• Filing date: Dec 10, 1986
• Grant date: May 30, 1989
• Expiry date: Dec 10, 2006

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/533
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a process for increasing the quantum yield in the case of the oxidation of luminol or of a 7-dialkylaminonaphthalene-1,2-dicarboxylic acid hydrazide, each alkyl radical of which contains up to 3 carbon atoms, by a peroxide compound in the presence of peroxidase ( POD), wherein the reaction is carried out in the presence of fluorescein, the concentration of the fluorescein being in a concentration range which gives a quantum yield which is greater than the sum of the quantum yields of the individual chemiluminescing materials. The present invention also provides a reagent for the determination of POD, wherein it contains luminol or a 7-dialkylaminonaphthalene-1,2-dicarboxylic acid hydrazide, each alkyl radical of which contains up to 3 carbon atoms, fluorescein, a hydrogen peroxide provider, a buffer substance (pH 7.5 to 9) and optionally a sequestering agent.