Bonded phase chromatographic supports
US4837348A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 30, 1986 |
| Grant date | Jun 6, 1989 |
| Priority date | — |
| Expiry date | Apr 30, 2006 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2220/58
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention relates to the functionalization of particulate bonded phase chromatographic supports prepared by silanization of silica gel or controlled pore glass and containing pendant primary alkyl amine groups. Functionalization results from the activation of the amines by reaction with N,N'-carbonyldiimidazole (CDI), or a related azolide, in anhydrous organic solvent, followed by derivatization of the support. Derivatization results from reaction of the activated support with a functionalizing reagent consisting of a primary or secondary, alkyl or aryl amine in organic solvent, or from an aqueous solution of the amine or its salt. A urea linkage results through which the functionalizing reagent is covalently attached to the support. Derivatization can result from the addition of an excess of a single reagent, or as a consequence of the sequential addition of two or more functionalizing reagents. Chromatographic supports preparation in this manner yield materials suitable for affinity, covalent, ion exchange and hydrophobic interaction chromatography of biomolecules as well as for the preparation of immobilized reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.