High sensitivity chromatography detector
US4838688A · kind A · utility
Inventor
Key dates
| Filing date | Oct 18, 1988 |
| Grant date | Jun 13, 1989 |
| Priority date | — |
| Expiry date | Oct 18, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/065
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A high sensitivity fluorescence and absorption detector comprising an elongated substantially cylindrical flow cell connected to and receiving the outflow of a fluid chromatography column; a mechanism for projecting a fluorescence generating beam of electromagnetic energy along on an axis substantially coincident with the axis of the flow cell; the flow cell including an elongated substantially cylindrical midsection housed by a tubular capillary transparent to the fluorescent radiation generated by the beam; a fluorescence integrating sphere completely surrounding the midsection of the capillary, the integrating sphere collecting at least about 3 pi steradian of the fluorescent radiation emanating through the capillary tube and into the sphere; the beam being projected to enter one end of the cylindrical flow cell, focus at about the center point of the axis of the cylindrical midsection, emanate through the other end of the flow cell and impinge upon a photodetector for detecting the portion of the beam which is not absorbed by the outflow flowing through the cell; the cross-sectional diameter and the axial length of the flow cell being selected such that the axially coincident b…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.