Method for immobilizing dissolved proteins

US4839419A · kind A · utility

Assignee

• Rohm Co., Ltd. · JP

Inventors

• Dieter Kraemer · Mainz, DE
• Hermann Plainer · Reinheim, DE
• Bruno Sproessier · Roßdorf, DE
• Helmut Uhlig · Roßdorf, DE
• Reiner Schnee · Darmstadt, DE

Key dates

• Filing date: Feb 16, 1988
• Grant date: Jun 13, 1989
• Expiry date: Feb 16, 2008

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A23C2220/104
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for adsorbing a protein, for example an enzyme, onto an insoluble, solid, macroporous, small-particle support by washing said support with an aqueous solution of the protein containing an electrolyte in an ionic strength of at least 0.15 mole/liter and crosslinking said protein, before, during, or after such adsorption, with a coupling component present in aqueous electrolyte-containing solution.