Patent · US Expired

Conjugate for enzyme immuno determinations

US4843001A · kind A · utility

5Cited by

2References

9Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Harald Haug · Peißenberg, DE
Gerd Kleinhammer · Tutzing, DE
Johann Mattersberger · Munich, DE

Key dates

Filing date	Jul 11, 1986
Grant date	Jun 27, 1989
Priority date	—
Expiry date	Jul 11, 2006

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/81
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

This invention teaches a process for preparing a conjugate useful in enzyme immunoassays, and the conjugate produced by the process. The process involves oxidizing an enzyme containing a carbohydrate portion, such as peroxidase, with periodic acid or an alkali metal salt thereof in an aqueous medium followed by reduction with sodium borohydride. The oxidation and reduction step may be carried out either before or after the enzyme is coupled to the immunologically effective component of the conjugate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.