Signal amplification in an assay employing a piezoelectric oscillator
US4847193A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 18, 1987 |
| Grant date | Jul 11, 1989 |
| Priority date | — |
| Expiry date | Jun 18, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/806
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting a first member of a specific binding pair in a sample, the first member being capable of forming a complex with a second member of the specific binding pair, includes immobilizing the second member on a surface of a piezoelectric oscillator, determining a first vibrational frequency of the oscillator on which the second member has been immobilized, contacting the surface of the oscillator on which the second member has been immobilized with the sample under conditions permitting formation of the complex, and with a particle under conditions permitting binding of the particle with the first member, mass coupling the bound particle with the oscillator, and determining a second vibrational frequency of the oscillator with which the particle has been mass coupled, the difference between the first vibrational frequency and the second vibrational frequency providing a measure of the quantity of the first member in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.