Lipase substrates

US4847376A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulrich Neumann · Peißenberg, DE
• Martina Junius · Bernried, DE
• Hans-Georg Batz · Tutzing, DE

Key dates

• Filing date: May 1, 1986
• Grant date: Jul 11, 1989
• Expiry date: May 1, 2006

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2334/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a lipase substrate of the general formula: ##STR1## wherein A is an alkylene or alkenylene radical containing up to 16 carbon atoms, R and R.sub.1, which can be the same or different, each signify an alkyl, alkenyl or acyl radical containing up to 20 carbon atoms or an optionally alkyl-substituted aryl or aralkyl radical containing up to 8 carbon atoms in the alkyl moiety and wherein one of R and R.sub.1 can also be a hydrogen atom, X is the residue of an aromatic hydroxy or thiol compound, and each Y and Z, independently from each other, is --S-- or --O--, Z also --CH.sub.2 --. The present invention also provides a process and a reagent for the optical determination of lipase.