Patent · US Expired

Process for solid phase-sequencing of nucleic acid fragments

US4849077A · kind A · utility

16Cited by

3References

6Claims

0Family size

Assignee

Akademie der Wissenchaften der DDR · DE

Inventors

Andre Rosenthal · Jena, DE
Hans-Dieter Hunger · Panketal, DE
Horst Kagelmaker · Panketal, DE
Monika Graatschus · Berlin, DE

Key dates

Filing date	Jul 31, 1985
Grant date	Jul 18, 1989
Priority date	—
Expiry date	Jul 31, 2005

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10T436/143333
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A process for solid phase sequencing of nucleic acid fragments is disclosed. The object of the invention is to provide a sequencing process which enables the simultaneous sequencing of large amounts of long and short nucleic acid fragments and which is optionally automated. Thus, a solid support combining mechanical stability, anion exchange characteristics, and chemical elution of nucleic acids off the support is used. Immobilized nucleic acid fragments are chemically modified and subsequently the nucleic acid backbone is cleaved and simultaneously or subsequently said fragments are eluted by chemical means. The present invention is applied to molecular biology and gene technology.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.