Process and reagent for the specific determination of HDL cholesterol in serum or plasma

US4851335A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Lorenz Kerscher · Penzberg, DE
• Joachim Siedel · Bernried, DE
• Joachim Ziegenhorn · Starnberg, DE
• Brigitte Pautz · Herrsching am Ammersee, DE

Key dates

• Filing date: Sep 16, 1986
• Grant date: Jul 25, 1989
• Expiry date: Sep 16, 2006

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/92
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement. The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains PA0 0.1 to 10 U/ml. cholesterol esterase PA0 0.05 to 10 U/ml. cholesterol oxidase PA0 20 to 500 mmole/liter buffer substance (pH 6.0 to 8.0) PA0 0.2 to 20 mmole…