Immunoaffinity purification system
US4851341A · kind A · utility
95Cited by
4References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 19, 1986 |
| Grant date | Jul 25, 1989 |
| Priority date | — |
| Expiry date | Dec 19, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is disclosed for purifying a recombinant fusion protein having an N-terminal identification sequence comprising multiple anionic amino acid residues, comprising forming a complex of the protein with a divalent cation dependent monoclonal antibody specific for the sequence, isolating the complex, and dissociating antibody and protein by selectively depleting the concentration of divalent cations in contact with the complex. A particular calcium-dependent monoclonal antibody, 4E11, is useful in an embodiment of the process which employs the identification peptide DYKDDDDK.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.