Continuous process for the enzymatic preparation of isomaltulose

US4857461A · kind A · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Peter Egerer · Wuppertal, DE
• Wulf Crueger · Erkrath, DE
• Gunter Schmidt-Kastner

Key dates

• Filing date: Apr 23, 1986
• Grant date: Aug 15, 1989
• Expiry date: Apr 23, 2006

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/88
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a continuous process for the enzymatic preparation of isomaltulose. A periplasmatic sucrose-mutase is produced by fermentation of microorganisms which form sucrose-mutase. The cell-free crude enzyme extract is prepared by digestion of the cells and by cross-flow microfiltration. In a single-stage, simultaneous purification and immobilization of the sucrose-mutase from the cell-free crude extract conditioned by diafiltration is obtained by selective bonding to an anionizable carrier matrix. Direct conversion of sucrose into isomaltulose is produced by the sucrose-mutase bonded to the anionizable carrier matrix, preferably in cartridge or cartouche form.