Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
US4859584A · kind A · utility
51Cited by
5References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 31, 1986 |
| Grant date | Aug 22, 1989 |
| Priority date | — |
| Expiry date | Oct 31, 2006 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5005
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for determining growth rate of cells growing in vivo and in vitro. Cells are labelled with cyanine dyes and changes in plasma membrane cyanine dye levels are used to determine growth rate. Cell growth rate determinations are utilized to monitor transplanted bone marrow cell engraftment and post-surgical corneal epitheal cell growth. The invented methods also are used to determine tumor cell sensitivity to cancer therapeutic agents, yeast sensitivity to antifungal agents, and bacteria sensitivity to antibacterial agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.