Method for preparing rabbit monoclonal antibodies, the cell lines used therein and the antibodies produced thereby
US4859595A · kind A · utility
Inventors
Key dates
| Filing date | May 3, 1985 |
| Grant date | Aug 22, 1989 |
| Priority date | — |
| Expiry date | May 3, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A new continuous rabbit cell line, designated TP-3, and variants thereof, which are hypoxyxanthineguanine-phosphoribosyl transferase deficient, hypoxyxanthine-aminopterine-thymidine sensitive, fast growing and non-antibody producing, and the methods for preparing the TP-3 cell line. Also, rabbit-rabbit hybrid cells formed by fusing the TP-3 cell line with antibody-producing cells from immunized rabbits, which hybrid cells are hypoxyxanthine-aminopterine-thymidine insensitve and secrete rabbit antibodies, and the methods for preparing the hybrid cells and hybrid cell lines. The invention further comprises rabbit monoclonal antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.