Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA
US4863582A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 28, 1988 |
| Grant date | Sep 5, 1989 |
| Priority date | — |
| Expiry date | Mar 28, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44756
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In an apparatus for purifying and concentrating DNA, from a crude mixture containing DNA, a unitary collection cup for collecting the DNA from a lysate sample is provided; also, a retainer member for retaining a gel matrix. Both the collection cup and the gel matrix, which is disposed above the collection cup, are adapted to be engaged with a lysate container. The container functions to receive the lysate sample, which sample typically is derived from human whole blood; rotation of the lysate sample container produces opening and closing of a pathway defined by slotted passages in the collection cup, thereby selectively controlling the flow of electrophoresis buffer solution through the interior of the collection cup at appropriate times.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.