Patent · US Expired

Double-stranded vector and process using it

US4863848A · kind A · utility

3Cited by

1References

6Claims

0Family size

Assignee

Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

Helmut Blocker · Braunschweig, DE
Ronald Frank · Lehre, DE
Guido Volckaert · Aarschot, BE

Key dates

Filing date	Apr 7, 1986
Grant date	Sep 5, 1989
Priority date	—
Expiry date	Apr 7, 2006

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/00
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention concerns a double-stranded recombinant DNA cloning vector comprising one single insertion site for foreign DNA and one or two labelling sites adjacent or approximate to said insertion site. In the instance of two labelling sites, said sites flank the insertion site. The labelling site(s) can be individually labelled in a fashion that results in the labelling of the 3' end of either strand. The use of the invention in a process affords the ability to individually label only one strand at the 3' end in a simplified method for base sequencing analysis.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.