Method of thioacylation peptide sequencing with alcoholysis of thiazolinones
US4863870A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1988 |
| Grant date | Sep 5, 1989 |
| Priority date | — |
| Expiry date | Mar 10, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/141111
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A sequencing method which exploits the thioacylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After sufficient time to insure quantitative coupling and removal of excess reagent, the N-thioacyl polypeptide is subjected to cleavage by acid which affords a 2-substituted-5(4H)-thiazolinone of the N-terminal amino acid. Subsequent addition of a large excess of an aliphatic primary or secondary alcohol, either directly to the cleavage acid or after its removal, yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and subsequent detection by contemporary methods of high pressure liquid chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.